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Your Position: ホーム > Protein > TIM-3 > TM3-H82E7

Biotinylated Human TIM-3 / HAVCR2 Protein, Avitag™,His Tag (MALS verified)

  • Synonym
    HAVCR2,TIM3,TIMD3,FLJ14428,KIM3
  • Source
    Biotinylated Human TIM-3, Avitag,His Tag(TM3-H82E7) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Arg 200 (Accession # Q8TDQ0-1).
    Predicted N-terminus: Ser 22
  • Molecular Characterization
    TIM-3 Structure

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 23.1 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TIM-3 SDS-PAGE

Biotinylated Human TIM-3, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
TIM-3 MALS images

The purity of Biotinylated Human TIM-3, Avitag,His Tag (Cat. No. TM3-H82E7) is more than 85% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.

Bioactivity-ELISA
 TIM-3 ELISA

Immobilized Anti-TIM3 MAb, Human IgG4 at 2 μg/mL (100 μL/well) can bind Biotinylated Human TIM-3, Avitag,His Tag (Cat. No. TM3-H82E7) with a linear range of 0.4-13 ng/mL (QC tested).

  • Background
    Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428,  KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.
  • Clinical and Translational Updates

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