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Human Integrin alpha 8 beta 1 (ITGA8&ITGB1) Heterodimer Protein, His Tag&Tag Free

  • シノニム
    Integrin alpha 8 beta 1,ITGA8&ITGB1
  • ソース
    Human ITGA8&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W9) is expressed from human 293 cells (HEK293). It contains AA Phe 39 - Leu 1012 (ITGA8) & Gln 21 - Asp 728 (ITGB1) (Accession # P53708-1 (ITGA8) & P05556-1 (ITGB1)).
    Predicted N-terminus: Phe 39 (ITGA8) & Gln 21 (ITGB1)
  • 分子特性
    Online(Phe 39 - Leu 1012 (ITGA8) & Gln 21 - Asp 728 (ITGB1)) P53708-1 (ITGA8) & P05556-1 (ITGB1)

    Human ITGA8&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA8 and ITGB1, has a calculated MW of 114.6 kDa (ITGA8) and 83.7 kDa (ITGB1). Subunit ITGA8 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 140-170 kDa (ITGA8) and 100-115 kDa (ITGB1) respectively due to glycosylation.

  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >95% as determined by SDS-PAGE.

  • 成分

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGA8&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W9) SDS-PAGE gel

Human ITGA8&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human ITGA8&ITGB1 Heterodimer Protein, His Tag&Tag FreeHuman ITGA8&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W9) ELISA bioactivity

Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGA8&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W9) with a linear range of 0.016-0.5 μg/mL (QC tested).

  • バックグラウンド
    Human Integrin alpha 8 beta 1 Heterodimer Protein consists of ITGA8 and ITGB1. The integrin alpha 8 subunit, isolated by low stringency hybridization, is a novel integrin subunit that associates with beta 1. The recently identified alpha 8 integrin subunit associates with beta 1 and is predominantly expressed in smooth muscle and other contractile cells in adult tissues, and in mesenchymal and neural cells during development. In addition, Integrin alpha 8 beta 1 is a receptor for fibronectin and can promote attachment, cell spreading, and neurite outgrowth on fibronectin.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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