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Your Position: ホーム > Protein > BTN3A1 > BT1-H82F7

Biotinylated Human BTN3A1 / CD277 Protein, Fc,Avitag™

  • シノニム
    BTN3A1,CD277,BTF5
  • ソース
    Biotinylated Human BTN3A1, Fc,Avitag (BT1-H82F7) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Gly 254 (Accession # O00481-1).
    Predicted N-terminus: Gln 30
  • 分子特性
    Online(Gln 30 - Gly 254) O00481-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 52.93 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • ビオチン化
    ビオチン化 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >95% as determined by SDS-PAGE.

  • 成分

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human BTN3A1, Fc,Avitag (Cat. No. BT1-H82F7) SDS-PAGE gel

Biotinylated Human BTN3A1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • バックグラウンド
    Butyrophilin subfamily 3 member A1 (BTN3A1) is also known as CD277 and BTF5, which belongs to the immunoglobulin superfamily and contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. Also, BTN3A1 regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. Furthermore, BTN3A1 mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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