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Your Position: ホーム > Protein > Her2 > HE2-H82E2

Biotinylated Human Her2 / ErbB2 Protein, His,Avitag™, premium grade

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    ERBB2,CD340,HER-2,neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    Biotinylated Human Her2, His,Avitag, premium grade(HE2-H82E2) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
    Predicted N-terminus: Thr 23
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
    Request for sequence
  • Molecular Characterization
    Her2 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 72.9 kDa. The protein migrates as 93 kDa±3 kDa under reducing (R) condition, and 85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Host Cell Protein
    <0.5 ng/µg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Sterility
    Negative
  • Mycoplasma
    Negative.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Her2 SDS-PAGE

Biotinylated Human Her2, His,Avitag, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Her2 MALS images

The purity of Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) is more than 90% and the molecular weight of this protein is around 70-100 kDa verified by SEC-MALS.

Report
Bioactivity-FACS
Her2 FACS

2e5 of anti-Her2 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

Protocol
Bioactivity-ELISA
Her2 ELISA

Immobilized Trastuzumab Biosimilar at 1 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) with a linear range of 0.05-2 ng/mL (QC tested).

Protocol
Her2 ELISA

Immobilized Pertuzumab Biosimilar at 1 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) with a linear range of 0.05-2 ng/mL (Routinely tested).

Protocol
Her2 ELISA

Immobilized Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Pertuzumab Biosimilar with a linear range of 0.05-2 ng/mL (Routinely tested).

Protocol
Bioactivity-SPR
Her2 SPR

Herceptin captured on Protein A Chip can bind Biotinylated Human Her2, His, Avitag, premium grade (Cat. No. HE2-H82E2) with an affinity constant of 30.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol
  • Background
    Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .
  • Clinical and Translational Updates

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