ログイン 会員登録
icon_bulk_order一括注文 Acrobiosystems for English
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
phone
Your Position: ホーム > Protein > FOLR1 > FO1-H82E2

Biotinylated Human FOLR1 Protein, His,Avitag™ (MALS verified)

  • Synonym
    FOLR-1,FBP,FOLR,FRα
  • Source
    Biotinylated Human FOLR1, His,Avitag(FO1-H82E2) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Met 233 (Accession # P15328-1).
    Predicted N-terminus: Arg 25
  • Molecular Characterization
    FOLR1 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 28.3 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FOLR1 SDS-PAGE

Biotinylated Human FOLR1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
FOLR1 MALS images

The purity of Biotinylated Human FOLR1, His,Avitag (Cat. No. FO1-H82E2) is more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
 FOLR1 ELISA

Immobilized Folic acid-BSA conjugate at 5 μg/mL (100 μL/well) can bind Biotinylated Human FOLR1, His,Avitag (Cat. No. FO1-H82E2) with a linear range of 0.004-0.125 μg/mL (QC tested).

Bioactivity-SPR
 FOLR1 SPR

Biotinylated Human FOLR1, His,Avitag (Cat. No. FO1-H82E2) immobilized on SA Chip can bind Folic acid-BSA with an affinity constant of 83.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-FACS
 FOLR1 FACS

2e5 of anti-FOLR1 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human FOLR1, His,Avitag (Cat. No. FO1-H82E2) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

  • Background
    Folate Receptor 1 (FOLR1) is also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.
  • Clinical and Translational Updates

コメント (0)


ETD of in-stock products: 4 business days

価格(JPY) : 103,040

価格(JPY) : 446,880

人気商品のおすすめ
ストレプトアビジンコートプレート

価格(JPY) : 8,960

価格(JPY) : 38,360

ストレプトアビジン-HRP

価格(JPY) : 14,000

価格(JPY) : 33,600

Magnetic Beads™ 磁気ビーズ

価格(JPY) : 38,640

価格(JPY) : 64,400

プロモーション&展示



医薬品開発状況

  • Number of Launched Drugs:3 Details
  • Number of Drugs in Clinical Trials:16 Details
  • Latest Research Phase:Approved

データシート&ドキュメンテーション

DMF請求
DMF (Drug Master File)

関連製品一覧

新製品

お問い合わせ&コメント

This web search service is supported by Google Inc.

totopphone