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Human Serum Albumin Protein, His Tag, low endotoxin (MALS verified)

  • シノニム
    Serum albumin,ALB,Alb
  • ソース
    Human Serum Albumin, His Tag (HSA-H522a) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Leu 609 (Accession # P02768-1).
    Predicted N-terminus: Asp 25
  • 分子特性
    Online(Asp 25 - Leu 609) P02768-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 67.4 kDa. The protein migrates as 67-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • エンドトキシン
    Less than 0.01 EU per μg by the LAL method.
  • 純度

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 成分

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Serum Albumin, His Tag (Cat. No. HSA-H522a) SDS-PAGE gel

Human Serum Albumin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Serum Albumin, His Tag (Cat. No. ) MALS images

The purity of Human Serum Albumin, His Tag (Cat. No. HSA-H522a) is more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) on SA Biosensor, can bind Human Serum Albumin, His Tag, low endotoxin (Cat. No. HSA-H522a) with an affinity constant of 0.596 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • バックグラウンド
    serum albumin (SA) is also known as ALB, which is the main protein of plasma and has a good binding capacity for water,Ca2+,Na+,K+,fatty acids,hormones, bilirubin and drugs.The main function of SA is the regulation of the colloidal osmotic pressure of blood. As Major zinc transporter in plasma, SA typically binds about 80% of all plasma zinc. A variant structure of albumin could lead to increased binding of zinc resulting in an asymptomatic augmentation of zinc concentration in the blood. Defects in serum albumin can cause familial dysalbuminemic hyperthyroxinemia which is a form of euthyroid hyperthyroxinemia that is due to increased affinity of serum albumin for T4. It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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