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Your Position: ホーム > Protein > Integrin alpha 5 beta 1 > IT1-H82Wa

Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, His,Avitag™&Tag Free

  • Synonym
    Integrin alpha 5 beta 1,ITGA5&ITGB1
  • Source
    Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free(IT1-H82Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1) (Accession # P08648-1 (ITGA5) & P05556-1 (ITGB1)).
    Predicted N-terminus: Phe 42 (ITGA5) & Gln 21 (ITGB1)
  • Molecular Characterization
    Integrin alpha 5 beta 1 Structure

    Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 112.4 kDa (ITGA5) and 83.7 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 100-115 kDa (ITGA5) and 135-150 kDa (ITGB1) respectively due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Integrin alpha 5 beta 1 SDS-PAGE

Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 Integrin alpha 5 beta 1 ELISA

Immobilized Recombinant Human Fibronectin at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) with a linear range of 1-16 ng/mL (QC tested).

Bioactivity-SPR
 Integrin alpha 5 beta 1 SPR

Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) captured on Biotin CAP-Series S Sensor Chip can bind Human Fibronectin with an affinity constant of 4.4 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Background
    Integrin alpha 5 beta 1 / Integrin α5β1, which is a major cellular receptor for the extracellular matrix protein fibronectin and plays a fundamental role during mammalian development., is composed of α5 (ITGA5/CD49e) and β1 (ITGB1/CD29) subunits. Integrins are adhesion receptors that transmit bidirectional signals across the plasma membrane. The Integrin alpha 5 beta 1 and its primary extracellular matrix ligand fibronectin (Fn) are of great biological importance.
  • Clinical and Translational Updates

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