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Human IL-5 Protein, His Tag

  • Synonym
    IL-5,TRF,IL5,Interleukin-5
  • Source
    Human IL-5 Protein, His Tag(IL5-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Ser 134 (Accession # P05113-1).
    Predicted N-terminus: His
  • Molecular Characterization
    IL-5 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 15.0 kDa. The protein migrates as 18-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Sterility
    Negative
  • Mycoplasma
    Negative.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-5 SDS-PAGE

Human IL-5 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 IL-5 ELISA

Immobilized Human IL-5 Protein, His Tag (Cat. No. IL5-H52H3) at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag (Cat. No. ILA-H5269) with a linear range of 0.6-10 ng/mL (QC tested).

Bioactivity-BLI
 IL-5 BLI

Loaded Human IL-5 Protein, His Tag (Cat. No. IL5-H52H3) on NTA Biosensor, can bind Human IL-5 R alpha, Fc Tag with an affinity constant of 4.63 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Bioactivity CELL BASE
 IL-5 CELL

Human IL-5 Protein, His Tag (Cat. No. IL5-H52H3) stimulates proliferation of Human IL-5 R alpha/CD131 (Luc) HEK293 Reporter Cell. The typically EC50 for this effect is 3.909 ng/mL (QC tested).

  • Background
    Interleukin 5 (IL5) is an interleukin produced by type-2 T helper cells and mast cells. IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely interleukin 3 and GM-CSF), this glycoprotein in its active form is a homodimer. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, wherein a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed. Given the high concordance of eosinophils and, in particular, allergic asthma pathology, it has been widely speculated that eosinophils have an important role in the pathology of this disease. Drugs that target IL-5 are mepolizumab and reslizumab.
  • Clinical and Translational Updates

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価格(JPY) : 98,000

価格(JPY) : 739,200

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