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Human VSIG3 Protein, Fc Tag

  • シノニム
    VSIG3,IgSF11,CXADRL1,Bt-IgSF,CT119
  • ソース
    Human VSIG3, Fc Tag (VS3-H5258) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Gly 241 (Accession # Q5DX21-1).
    Predicted N-terminus: Leu 23
  • 分子特性
    Online(Leu 23 - Gly 241) Q5DX21-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 49.7 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >95% as determined by SDS-PAGE.

  • 成分

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human VSIG3, Fc Tag (Cat. No. VS3-H5258) SDS-PAGE gel

Human VSIG3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human VSIG3, Fc TagHuman VSIG3, Fc Tag (Cat. No. VS3-H5258) ELISA bioactivity

Immobilized Human VSIG3, Fc Tag (Cat. No. VS3-H5258) at 5 μg/mL (100 μL/well) can bind Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B75-H5258) with a linear range of 0.313-10 μg/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B75-H5258) Captured on CM5 chip via anti-mouse antibodies surface can bind Human VSIG3, Fc Tag (Cat. No. VS3-H5258) with an affinity constant of 8.87 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Human VSIG3, Fc Tag (Cat. No. VS3-H5258) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human B7-H5, His Tag (Cat. No. B75-H52H0) with an affinity constant of 53.4 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human VSIG3, Fc Tag (Cat. No. VS3-H5258) on AHC Biosensor, can bind Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B75-H5258) with an affinity constant of 3.7 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human B7-H5, Avitag,His Tag (Cat. No. B75-H82E1) on SA Biosensor, can bind Human VSIG3, Fc Tag (Cat. No. VS3-H5258) with an affinity constant of 0.312 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human B7-H5, Fc,Avitag (Cat. No. B75-H82F3) on SA Biosensor, can bind Human VSIG3, Fc Tag (Cat. No. VS3-H5258) with an affinity constant of 0.617 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • バックグラウンド
    VSIG3, also known as IGSF11, BT-IgSF, and CLMP, is a homophilic adhesion molecule that preferentially expressed in the brain. The function of VSIG3 is to stimulate cell growth through homophilic interactions. In clinical, the VSIG3 has been reported to as a novel target for cancer immunotherapy of gastrointestinal and hepatocellular carcinomas. In addition, VSIG-3 is also a ligand of B7 family member VISTA/PD-1H and inhibits human T-cell functions through a novel VSIG-3/VISTA pathway. VSIG-3/VISTA co-inhibitory pathway may provide new strategies for the treatment of human cancers and autoimmune disorders.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETA of in-stock products: 4 business days

価格(JPY) : 98,000

価格(JPY) : 711,200

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