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Your Position: ホーム > Protein > CD4 > CD4-HF2H7

FITC-Labeled Human CD4 Protein, His Tag

  • Synonym
    CD4,CD4mut,LEU3
  • Source
    FITC-Labeled Human CD4, His Tag (CD4-HF2H7) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # AAH25782). It is the FITC labeled form of Human CD4, His Tag (LE3-H5228).
    Predicted N-terminus: Lys 26
  • Molecular Characterization
    CD4 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 41.5 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Protein Ratio
    The FITC to protein molar ratio is 3-5.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD4 SDS-PAGE

FITC-Labeled Human CD4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 CD4 ELISA

Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD4, His Tag (Cat. No. CD4-HF2H7) with a linear range of 0.01-0.156 μg/mL (QC tested).

 CD4 ELISA

Immobilized HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (Cat. No. GP5-V15224) at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human CD4, His Tag (Cat. No. CD4-HF2H7) with a linear range of 0.039-0.625 μg/mL (Routinely tested).

Bioactivity-FACS
 CD4 FACS

2e5 of CD4-CAR-293 cells were stained with 100 μL of 0.3 μg/mL of FITC-Labeled Human CD4, His Tag (Cat. No. CD4-HF2H7) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).

  • Background
    T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.
  • Clinical and Translational Updates

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