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Your Position: ホーム > Protein > IL-31 RA > ILA-H52H7

Human IL-31 RA Protein, His Tag (MALS & SPR verified)

  • Synonym
    IL-31 RA,IL-31RA,hGLM-R,CRL3,GPL,ZcytoR17,GLM-R,IL-31R-alpha,Gp130-like receptor
  • Source
    Human IL-31 RA, His Tag (ILA-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 519 (Accession # Q8NI17-1).
    Predicted N-terminus: Ala 20
  • Molecular Characterization
    Online(Ala 20 - Glu 519) Q8NI17-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 59.0 kDa. The protein migrates as 90-110 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-31 RA, His Tag (Cat. No. ILA-H52H7) SDS-PAGE gel

Human IL-31 RA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human IL-31 RA, His Tag (Cat. No. ) MALS images

The purity of Human IL-31 RA, His Tag(Cat. No. ILA-H52H7) is more than 90% and the molecular weight of this protein is around 80-100 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human IL-31, His Tag (Cat. No. IL1-H5247) immobilized on CM5 Chip can bind Human IL-31 RA, His Tag (Cat. No. ILA-H52H7) with an affinity constant of 52.1 nM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    Interleukin-31 receptor subunit alpha is a protein that in humans is encoded by the IL31RA gene, also known as IL-31 receptor subunit alpha, IL-31RA, GLM-R, Gp130-like receptor, CRL3, GPL. Oncostatin M receptor (OSMR) and IL31RA form the heterodimeric receptor through which IL31 signals. IL31RA is a strong activator of STAT3 and STAT5, whereas STAT1 is only marginally tyrosine-phosphorylated. dditionally, demonstrate Jak1 binding to GPL and its activation in heteromeric complexes with the OSMRbeta but also in a homomeric receptor complex.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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