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Your Position: ホーム > Protein > BAFFR > BAR-C52H4

Cynomolgus BAFFR / TNFRSF13C Protein, His Tag (MALS verified)

  • Synonym
    BAFFR,TNFRSF13C,BROMIX,CD268,CVID4,prolixin,BAFF-R
  • Source
    Cynomolgus BAFFR, His Tag(BAR-C52H4) is expressed from human 293 cells (HEK293). It contains AA Ser 7 - Gly 76 (Accession # XP_005567184.1).
  • Molecular Characterization
    BAFFR Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 9.1 kDa. The protein migrates as 14-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
BAFFR SDS-PAGE

Cynomolgus BAFFR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
BAFFR MALS images

The purity of Cynomolgus BAFFR, His Tag (Cat. No. BAR-C52H4) is more than 85% and the molecular weight of this protein is around 10-20 kDa verified by SEC-MALS.

Bioactivity-ELISA
 BAFFR ELISA

Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 1 μg/mL (100 μL/well) can bind Cynomolgus BAFFR, His Tag (Cat. No. BAR-C52H4) with a linear range of 0.4-3 ng/mL (QC tested).

 BAFFR ELISA

Immobilized Cynomolgus BAFFR, His Tag (Cat. No. BAR-C52H4) at 1 μg/mL (100 μL/well) can bind Anti-BAFFR Antibody, Human IgG1 with a linear range of 0.1-0.8 ng/mL (Routinely tested).

 BAFFR ELISA

Immobilized Cynomolgus BAFFR, His Tag (Cat. No. BAR-C52H4) at 1 μg/mL (100 μL/well) can bind Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) with a linear range of 0.1-2 ng/mL (Routinely tested).

  • Background
    BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), is a membrane protein of the TNF receptor superfamily which recognizes BAFF. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of BAFF in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.
  • Clinical and Translational Updates

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