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Your Position: ホーム > Protein > Flt-3 > FL3-H52H6

Human Flt-3 / Flk-2 Protein, His Tag (MALS verified)

  • Synonym
    Flt-3,Flk-2,STK-1,CD135,FLK2,FLT-3
  • Source
    Human Flt-3, His Tag(FL3-H52H6) is expressed from human 293 cells (HEK293). It contains AA Asn 27 - Ser 543 (Accession # P36888-1).
  • Molecular Characterization
    Flt-3 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 60.4 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Flt-3 SDS-PAGE

Human Flt-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Flt-3 MALS images

The purity of Human Flt-3, His Tag (Cat. No. FL3-H52H6) is more than 90% and the molecular weight of this protein is around 78-95 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Flt-3 ELISA

Immobilized Human Flt-3 Ligand, premium grade (Cat. No. FLL-H5218) at 10 μg/mL (100 μL/well) can bind Human Flt-3, His Tag (Cat. No. FL3-H52H6) with a linear range of 0.078-1.25 μg/mL (QC tested).

  • Background
    Flt-3(Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2(Fetal liver kinase-2), STK-1(Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
  • Clinical and Translational Updates

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価格(JPY) : 84,000

価格(JPY) : 610,400

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