ログイン 会員登録
icon_bulk_order一括注文 Acrobiosystems for English
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
phone
Your Position: ホーム > Protein > Fc gamma RIIIA / CD16a > CDA-H5220

Human Fc gamma RIIIA / CD16a (F176) Protein, His Tag (SPR & BLI & MALS verified)

  • Synonym
    FCGR3A,CD16A,FCG3,FCGR3,IGFR3
  • Source
    Human CD16a (F176), His Tag(CDA-H5220) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1).
    Predicted N-terminus: Gly 17
  • Molecular Characterization
    Fc gamma RIIIA / CD16a Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 23.7 kDa. The protein migrates as 39-51 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Fc gamma RIIIA / CD16a SDS-PAGE

Human CD16a (F176), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Fc gamma RIIIA / CD16a MALS images

The purity of Human CD16a (F176), His Tag (Cat. No. CDA-H5220) is more than 90% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.

Bioactivity-SPR
 Fc gamma RIIIA / CD16a SPR

Immobilized Human CD16a (F176), His Tag (Cat. No. CDA-H5220) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 2.70 μM as determined in a SPR assay (Biacore 8K) (QC tested).

 Fc gamma RIIIA / CD16a SPR

Rituximab immobilized on CM5 Chip can bind Human CD16a (F176), His Tag (Cat. No. CDA-H5220) with an affinity constant of 1.82 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
 Fc gamma RIIIA / CD16a BLI

Loaded Human CD16a (F176), His Tag (Cat. No. CDA-H5220) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 1.72 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Fc gamma RIIIA / CD16a BLI

Loaded Herceptin on Protein A Biosensor, can bind Human CD16a (F176), His Tag (Cat. No. CDA-H5220) with an affinity constant of 0.47 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Fc gamma RIIIA / CD16a BLI

Loaded Herceptin on FAB2G Biosensor, can bind Human CD16a (F176), His Tag (Cat. No. CDA-H5220) with an affinity constant of 0.51 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages.
    CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
  • Clinical and Translational Updates

コメント (0)


ETD of in-stock products: 4 business days

価格(JPY) : 61,600

価格(JPY) : 78,400

価格(JPY) : 554,400

人気商品のおすすめ
抗His抗体

価格(JPY) : 67,200

価格(JPY) : 442,400

コンジュゲート HRP Anti-His 抗体

価格(JPY) : 70,000

価格(JPY) : 470,400

プロモーション&展示



医薬品開発状況

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:12 Details
  • Latest Research Phase:Phase 2 Clinical

データシート&ドキュメンテーション

DMF請求
DMF (Drug Master File)

関連製品一覧

新製品

お問い合わせ&コメント

This web search service is supported by Google Inc.

totopphone