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Human TFPI Protein, His Tag (active enzyme)

  • シノニム
    TFPI,LACI,TFPI1,EPI,TFI
  • ソース
    Human TFPI, His Tag (TFI-H5226) is expressed from human 293 cells (HEK293). It contains AA Asp 29 - Lys 282 (Accession # NP_006278.1).
    Predicted N-terminus: Asp 29
  • 分子特性
    Online(Asp 29 - Lys 282) NP_006278.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 30.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >90% as determined by SDS-PAGE.

  • 成分

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human TFPI, His Tag (Cat. No. TFI-H5226) SDS-PAGE gel

Human TFPI, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

生物活性

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate,Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate. The IC50 value is <0.50 nM, as measured under the described conditions (QC tested).

  • バックグラウンド
    Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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