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Your Position: ホーム > Protein > CD5 > CD5-HF255

FITC-Labeled Human CD5 Protein, Fc Tag (MALS verified)

  • Synonym
    CD5,LEU1
  • Source
    FITC-Labeled Human CD5, Fc Tag(CD5-HF255) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Pro 372 (Accession # P06127-1).
    Predicted N-terminus: Arg 25
  • Molecular Characterization
    CD5 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 65.1 kDa. The protein migrates as 75-95 kDa and >116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD5 SDS-PAGE

FITC-Labeled Human CD5, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
CD5 MALS images

The purity of FITC-Labeled Human CD5, Fc Tag (Cat. No. CD5-HF255) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD5 ELISA

Immobilized FITC-Labeled Human CD5, Fc Tag (Cat. No. CD5-HF255) at 1 μg/mL (100 μL/well) can bind Mouse Anti-CD5 Antibody, Mouse IgG1 with a linear range of 0.2-3 ng/mL (QC tested).

Bioactivity-FACS
 CD5 FACS

5e5 of Anti-CD5 CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human CD5, Fc Tag (Cat. No. CD5-HF255) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).

  • Background
    T-cell surface glycoprotein CD5 is also known as Lymphocyte antigen T1/Leu-1 and LEU1,which is phosphorylated on tyrosine residues by LYN,so CD5 can create binding sites for PTPN6/SHP-1.CD5 may act as a receptor in regulating T-cell proliferation. CD5 is expressed at various developmental and activation stages on human B cells.CD5 is a well established negative regulator of TCR and BCR signalling.CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. Development,selection and function of different B- and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules.
  • Clinical and Translational Updates

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