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Your Position: ホーム > Protein > IL-1 RAcP > ILP-H82E5

Biotinylated Human IL-1 RAcP / IL-1 R3 Protein, His,Avitag™ (MALS verified)

  • Synonym
    IL1RAP,IL-1RAcP,C3orf13,IL1R3
  • Source
    Biotinylated Human IL-1 RAcP, His,Avitag(ILP-H82E5) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Glu 359 (Accession # Q9NPH3-1).
    Predicted N-terminus: Ser 21
  • Molecular Characterization
    IL-1 RAcP Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 42.7 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-1 RAcP SDS-PAGE

Biotinylated Human IL-1 RAcP, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-1 RAcP MALS images

The purity of Biotinylated Human IL-1 RAcP, His,Avitag (Cat. No. ILP-H82E5) is more than 90% and the molecular weight of this protein is around 50-68 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-1 RAcP ELISA

Immobilized Biotinylated Human IL-1 RAcP, His,Avitag (Cat. No. ILP-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Anti-IL1RAP Human IgG1 with a linear range of 0.1-1 ng/mL (QC tested).

  • Background
    Interleukin-1 receptor accessory protein (IL1RAP or IL-1RAcP) is also known as Interleukin-1 receptor 3 (IL-1R-3 or IL-1R3), C3orf13, which belongs to the interleukin-1 receptor family. IL1RAP contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RAP is detected in liver, skin, placenta, thymus and lung. IL-1R3 / IL1RAP is coreceptor with IL1R1, associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.
  • Clinical and Translational Updates

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