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Your Position: ホーム > Protein > MMP-7 > MM7-H5249

Human MMP-7 / PUMP1 Protein, His Tag (active enzyme)

  • Synonym
    MMP7,Matrilysin,Matrin,MPSL1,PUMP1
  • Source
    Human MMP-7, His Tag (MM7-H5249) is expressed from human 293 cells (HEK293). It contains AA Leu 18 - Lys 267 (Accession # P09237-1). It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.
    Predicted N-terminus: Leu 18
  • Molecular Characterization
    MMP-7 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 29.7 kDa. The protein migrates as 30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 10 mM HEPES,150 mM NaCl,pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
MMP-7 SDS-PAGE

Human MMP-7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 MMP-7 ELISA

Immobilized Human MMP-7, His Tag (Cat. No. MM7-H5249) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-MMP7 Polyclonal Antibody (Routinely tested).

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is >600 pmol/min/μg (QC tested).

  • Background
    Matrix metalloproteinase-7(MMP-7)also known as neutrophil collagenase and CLG1,is a member of matrix metalloproteinases (MMPs) family,which degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes as well as pathological processes. MMP-7 may affect the metastatic behavior of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-7 in the tumor may have a protective effect against lymph node metastasis.
  • Clinical and Translational Updates

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