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Your Position: ホーム > Protein > C1q R1 > C11-H82E9

Biotinylated Human C1q R1 / CD93 Protein, His,Avitag™

  • Synonym
    CD93,CDw93,C1QR1,MXRA4,C1qR,C1qR(P),C1qRP,ECSM3
  • Source
    Biotinylated Human C1q R1, His,Avitag(C11-H82E9) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Lys 580 (Accession # Q9NPY3-1).
    Predicted N-terminus: Thr 22
  • Molecular Characterization
    C1q R1 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 62.0 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
C1q R1 SDS-PAGE

Biotinylated Human C1q R1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 C1q R1 ELISA

Immobilized Human IGFBP-7, Fc Tag (Cat. No. IG7-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human C1q R1, His,Avitag (Cat. No. C11-H82E9) with a linear range of 1-31 ng/mL (QC tested).

Bioactivity-BLI
 C1q R1 BLI

Loaded Biotinylated Human C1q R1, His,Avitag (Cat. No. C11-H82E9) on SA Biosensor, can bind Human IGFBP-7, His Tag (Cat. No. IG7-H5240) with an affinity constant of 0.93 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    CD antigen CD93 is also known as Complement component C1q receptor (C1QR1). which belongs to the Group XIV C-Type lectin family, a group containing two other members, endosialin (CD248) and thrombomodulin. CD93 contains one C-type lectin domain and five EGF-like domains. CD93 is highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. CD93 is receptor for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). CD93 may mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens and may play a role in intercellular adhesion.
  • Clinical and Translational Updates

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