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Your Position: ホーム > Protein > NFL > NFL-H5143

Human NFL Protein, His Tag

  • Synonym
    Neurofilament light polypeptide
  • Source
    Human NFL, His Tag(NFL-H5143) is expressed from E. coli cells. It contains AA Ser 2 - Asp 543 (Accession # P07196-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    NFL Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 63.4 kDa.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 12 months under sterile conditions.
SDS-PAGE
NFL SDS-PAGE

The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 NFL ELISA

Immobilized Human NFL, His Tag (Cat. No. NFL-H5143) at 1 μg/mL (100 μL/well) can bind Anti NEFL Antibody with a linear range of 1-16 ng/mL (QC tested).

  • Background
    Neurofilaments (NF) are classed as type IV intermediate filaments found in the cytoplasm of neurons. Together with microtubules and microfilaments, they form the neuronal cytoskeleton. Neurofilaments usually contain three intermediate filament proteins: NFL, NFM, and NFH which are involved in the maintenance of neuronal caliber. Neurofilament heavy (NFH) can server as biomarkers for several diseases by indicating axonal injury.
  • Clinical and Translational Updates

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