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Your Position: ホーム > Protein > GFAP > GFP-M5148

Mouse GFAP Protein, His Tag

  • Synonym
    Glial fibrillary acidic protein
  • Source
    Mouse GFAP, His Tag(GFP-M5148) is expressed from E. coli cells. It contains AA Met 1 - Met 430 (Accession # P03995-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    GFAP Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 51.9 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 0.5 M Arginine, 150 mM NaCl, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
GFAP SDS-PAGE

Mouse GFAP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 GFAP ELISA

Immobilized Mouse GFAP, His Tag (Cat. No. GFP-M5148) at 1 μg/mL (100 μL/well) can bind GFAP Monoclonal Antibody with a linear range of 0.4-25 ng/mL (QC tested).

  • Background
    GFAP (Glial fibrillary acidic protein) is a monomeric intermediate filament protein fuond in mature astrocytes and other glial cells but is not found outside the CNS. Increased GFAP immunoreactivity (or astrocytic activation) is usually viewed as an index of gliosis or a relatively slow-developing correlate of neural damage.
  • Clinical and Translational Updates

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