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Human GFAP Protein, His Tag

  • Synonym
    Glial fibrillary acidic protein
  • Source
    Human GFAP, His Tag(GFP-H5143) is expressed from E. coli cells. It contains AA Met 1 - Met 432 (Accession # P14136-1 ).
    Predicted N-terminus: Met
  • Molecular Characterization
    GFAP Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 51.9 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, 0.5 M Arginine, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
GFAP SDS-PAGE

Human GFAP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 GFAP ELISA

Immobilized Human GFAP, His Tag (Cat. No. GFP-H5143) at 1 μg/mL (100 μL/well) can bind GFAP Monoclonal Antibody with a linear range of 0.8-25 ng/mL (QC tested).

  • Background
    GFAP (Glial fibrillary acidic protein) is a monomeric intermediate filament protein fuond in mature astrocytes and other glial cells but is not found outside the CNS. Increased GFAP immunoreactivity (or astrocytic activation) is usually viewed as an index of gliosis or a relatively slow-developing correlate of neural damage.
  • Clinical and Translational Updates

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