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Your Position: ホーム > Protein > Podoplanin > PON-H5254

Human Podoplanin / PDPN Protein, Fc Tag (MALS verified)

  • Synonym
    Podoplanin,PDPN,Gp36
  • Source
    Human Podoplanin, Fc Tag(PON-H5254) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Leu 131 (Accession # Q86YL7-1).
    Predicted N-terminus: Ala 23
  • Molecular Characterization
    Podoplanin Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 37.6 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Podoplanin SDS-PAGE

Human Podoplanin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Podoplanin MALS images

The purity of Human Podoplanin, Fc Tag (Cat. No. PON-H5254) is more than 90% and the molecular weight of this protein is around 90-110 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Podoplanin ELISA

Immobilized Human CLEC-2, His Tag (Cat. No. CL2-H5247) at 5 μg/mL (100 μL/well) can bind Human Podoplanin, Fc Tag (Cat. No. PON-H5254) with a linear range of 0.3-20 ng/mL (QC tested).

  • Background
    Podoplanin (PDPN) is a type I transmembrane mucin-like glycoprotein expressed in the lymphatic endothelium. It is overexpressed in several solid tumors, such as squamous cell carcinoma, malignant mesothelioma, Kaposi sarcoma, angiosarcoma, testicular seminoma, and brain tumors. PDPN expression is associated with malignant progression, epithelial–mesenchymal transition, and metastasis.
  • Clinical and Translational Updates

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