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Human GLP1R Protein, His Tag (MALS verified)

  • Synonym
    GLP-1-R, GLP-1R, glucagon like peptide 1 receptor
  • Source
    Human GLP1R Protein, His Tag(GLR-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Glu 139 (Accession # P43220-1).
    Predicted N-terminus: Ala 21
  • Molecular Characterization
    GLP1R Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 15.6 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
GLP1R SDS-PAGE

Human GLP1R Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
GLP1R MALS images

The purity of Human GLP1R Protein, His Tag (Cat. No. GLR-H52H7) is more than 90% and the molecular weight of this protein is around 28-38 kDa verified by SEC-MALS.

Bioactivity-ELISA
 GLP1R ELISA

Immobilized Human GLP1R Protein, His Tag (Cat. No. GLR-H52H7) at 2 μg/mL (100 μL/well) can bind Mouse Monoclonal GLP-1R Antibody with a linear range of 0.2-4 ng/mL (QC tested).

  • Background
    G-protein coupled receptor for glucagon-like peptide 1 (GLP-1). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels. Plays a role in regulating insulin secretion in response to GLP-1
  • Clinical and Translational Updates

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