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Your Position: ホーム > Antibody > His > HIS-BVM539

Biotinylated Anti-His Tag Antibody(AY63), Fab (MALS verified)

  • Source
    Biotinylated Anti-His Tag Antibody(AY63), Fab is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
  • Clone

    AY63

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Biotin

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Tag

  • Immunogen

    A protein peptide with his tag.

  • Specificity

    This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

  • Application
    ApplicationRecommended Usage
    ELISA0.1-100 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >98% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70 °C for 3 months after reconstitution;
SDS-PAGE
His SDS-PAGE

Biotinylated Anti-His Tag Antibody(AY63), Fab on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
His MALS images

The purity of Biotinylated Anti-His Tag Antibody(AY63), Fab (Cat. No. HIS-BVM539) is more than 98% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.

Bioactivity-ELISA
 His ELISA

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (BQ.1/Omicron) (Cat. No. NUN-C52Hx) at 1μg/mL (100μL/well) can bind Biotinylated Anti-His Tag Antibody(AY63), Fab (Cat. No. HIS-BVM539) with a linear range of 0.2-100ng/mL (QC tested).

  • Background
    His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 70,000

価格(JPY) : 470,400

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