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Your Position: ホーム > Protein > CD4 > CD4-R82E3

Biotinylated Rhesus macaque CD4 Protein, His,Avitag™ (MALS verified)

  • Synonym
    CD4,CD4mut,LEU3
  • Source
    Biotinylated Rhesus macaque CD4,His,Avitag(CD4-R82E3) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # G7N5T8-1).
    Predicted N-terminus: Lys 26
  • Molecular Characterization
    CD4 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 44.0 kDa. The protein migrates as 52-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD4 SDS-PAGE

Biotinylated Rhesus macaque CD4,His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
CD4 MALS images

The purity of Biotinylated Rhesus macaque CD4,His,Avitag (Cat. No. CD4-R82E3) is more than 90% and the molecular weight of this protein is around 43-53 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD4 ELISA

Immobilized Anti-CD4 Antibody (Ibalizumab-IgG4) at 1 μg/mL (100 μL/well) can bind Biotinylated Rhesus macaque CD4,His,Avitag (Cat. No. CD4-R82E3) with a linear range of 0.5-16 ng/mL (QC tested).

 CD4 ELISA

Immobilized Biotinylated Rhesus macaque CD4,His,Avitag (Cat. No. CD4-R82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-CD4 Antibody (Ibalizumab-IgG4) with a linear range of 0.1-2 ng/mL (Routinely tested).

  • Background
    T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.
  • Clinical and Translational Updates

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