ログイン 会員登録
icon_bulk_order一括注文 Acrobiosystems for English
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
phone
Your Position: ホーム > Protein > Beta-NGF > BEF-H5214

Human Beta-NGF Protein, premium grade

  • Synonym
    NGF,NGFB,Beta-NGF,HSAN5,MGC161426,MGC161428
  • Source
    Human Beta-NGF Protein, premium grade(BEF-H5214) is expressed from human 293 cells (HEK293). It contains AA Ser 122 - Ala 241 (Accession # P01138).
    Predicted N-terminus: Ser 122
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization
    Beta-NGF Structure

    This protein carries no "tag".

    The protein has a calculated MW of 13.5 kDa. The protein migrates as 14-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Sterility
    Negative
  • Mycoplasma
    Negative.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM NaAC, 150 mM NaCl, pH5.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Beta-NGF SDS-PAGE

Human Beta-NGF Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Beta-NGF MALS images

The purity of Human Beta-NGF Protein, premium grade (Cat. No. BEF-H5214) is more than 90% and the molecular weight of this protein is around 32-50 kDa verified by SEC-MALS.

Bioactivity-Bioactivity CELL BASE
 Beta-NGF CELL

Human Beta-NGF Protein, premium grade (Cat. No. BEF-H5214) stimulates proliferation of TF-1 Cells. The typically EC50 for this effect is 2.32 ng/mL (QC tested).

Bioactivity-ELISA
 Beta-NGF ELISA

Immobilized Human Beta-NGF Protein, premium grade (Cat. No. BEF-H5214) at 1 μg/mL (100 μL/well) can bind Human TrkA (33-417), Mouse IgG2a Fc Tag (Cat. No. TRA-H5259) with a linear range of 1-16 ng/mL (QC tested).

 Beta-NGF ELISA

Immobilized Human Beta-NGF Protein, premium grade (Cat. No. BEF-H5214) at 1 μg/mL (100 μL/well) can bind Human TrkA (192-402), Mouse IgG2a Fc Tag (Cat. No. TRA-H5253) with a linear range of 1-16 ng/mL (Routinely tested).

  • Background
    Nerve growth factor beta (beta-NGF) is a neurotrophic factor that plays critical role in the development and maintenance of sensory and sympathetic neurons. The active form of human beta-NGF is is a noncovalently disulfide-linked homodimer, which is secreted and signals through the low affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase A (TrkA) to activate PI3K, Ras, and PLC signaling pathways.
  • Clinical and Translational Updates

コメント (0)


ETD of in-stock products: 4 business days

価格(USD) : 88.00

価格(USD) : 130.00

価格(USD) : 780.00

プロモーション&展示



医薬品開発状況

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:13 Details
  • Latest Research Phase:NDA/BLA

データシート&ドキュメンテーション

DMF請求
DMF (Drug Master File)

関連製品一覧

新製品

お問い合わせ&コメント

This web search service is supported by Google Inc.

totopphone