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Here come GMP Grade Cytokines!Free Sample is available! Here come GMP Grade Cytokines!Free Sample is available!
Assay Type | FRET |
Analyte | DNase I |
Format | 96T/480T |
Regulatory Status | RUO |
Sensitivity | 3.9×10⁻⁵ U |
Standard Curve Range | 0.0025 U-3.9×10⁻⁵ U |
Assay Time | 30 min |
Suitable Sample Type | For the quantitative determination of DNase I in the environment, some biological materials, common molecular biological reagents such as reaction buffers. |
Sample volume | 10 μL & 80 μL |
Items | Size (96tests) | Size(480tests) |
DNase Substrate | 2 nmol | 10 nmol |
10X Reaction Buffer for DNase | 10 mL | 10 mL |
DNase I (1U/μL) | 20 μL | 50 μL |
TE Buffer (pH 8.0) | 1.5 mL | 6 mL |
Nuclease-free Water | 10 mL | 50 mL |
DNases are ubiquitous in both the environment and many biological materials. Many molecular biology experiments rely on the use of plastics, chemicals, and solutions that are free from detectable DNase activity, once these materials are contaminated with DNases, the experiments will be affected because of the DNases can degrade DNA. Since even only minute amounts of DNase contamination would ruin the experiment, it is necessary to evaluate the presence of DNase with a reasonable method.
Published methods for detecting DNase such as Nucleic acid hydrolyzed gel electrophoresis and ultraviolet spectrophotometer are typically time consuming, not quantitative, and relatively insensitive. The other methods such as HPLC and electrochemical methods are limited to instrumentation.
In contrast, the DNase Activity Assay Kit (Fluorescence) can detect DNase contamination within 30 minutes, and the kit is high sensitivity, easy to use. Moreover, the DNase and RNase Activity Assay Kit (Fluorescence) have been designed to work together seamlessly for simultaneous quantitative detection of DNases and RNases in a single sample.
The DNase Activity Assay Kit (Fluorescence) is a convenient and sensitive assay tool to test the presence of DNase in buffers, reagents, and other components.
It is for research use only.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 3 months from the date of opening.
Add 90 μL of the working DNase Substrate solution to each 96-well plate, and add 10 μL of DNase I standards (0.00000390625-0.00025 U/μL*10μL/well = 0.0000390625-0.0025U/well), incubate the plate in the fluorometer (BMG CLARIOstar) collecting real-time data at 1 minute intervals for 30 minutes at 37°C using the settings described in this section. The DNase Activity Assay can be evaluated in rigorous kinetic terms using real-time data.
This assay kit employs a standard detection of DNase I. Add 90 μL working DNase Substrate solution to each 96-well plate, and add 10 μL DNase I standards ((0.00000390625-0.00025 U/μL*10μL/well = 0.0000390625-0.0025U/well), incubate for 30 minutes at 37°C. Then measure the fluorescence using the settings described in this section in a fluorometer (BMG CLARIOstar). Take RFU of standards as the ordinate and DNase concentration as the abscissa. Four parameters logistic are used to draw the standard curve (QC tested).
Eight replicates of each of seven samples containing different concentrations of DNase were tested in one assay, Intra-Assay Precision CV<10%.
Eight replicates of each of seven samples containing different concentrations of DNase were tested in eight independent assays, Inter-Assay Precision CV<15%.
The probe is freeze-thawed 3 times, the kit performance meets the requirements, the sensitivity is not reduced, and the CV< 10%.
Three different concentrations of DNase was spiked into four different kinds of matrixes. The range of the recovery rate is 80%~120%.
DNase Activity Assay Kit (Fluorescence) (Cat. No. ASE-A002) and RNase Activity Assay Kit (Fluorescence) (Cat. No. ASE-A001) were used to detect nuclease residues in the same sample. No significant cross-reactivity or interference was observed.
ACROBiosystemsは、二重特異性抗体の治療薬への臨床開発を加速するために、高い生物活性を持つ均一なCD3δ/CD3εおよびCD3γ/CD3εタンパク質のシリーズを開発しました。
We offer a wide range of cell and gene therapy solutions starting from discovery to the clinic. Explore our wide range of proteins, antibodies, kits, and other assays to accelerate the development of your cell and gene therapy.
Organoid Toolbox は、即使用可能のオルガノイド、オルガノイド分化ツールキット、創薬プロジェクトの進行を加速する、さまざまなサービスを含むオルガノイドソリューションのことです。
IL-15、IL-7、IL-21など、高品質のGMP準拠サイトカイン製品を提供しており、免疫細胞治療薬の臨床研究をサポートし、医薬品規制当局の承認を加速できます。
注目されている50以上のCAR-Tターゲットがあり、CARの発現の検出のために設計された抗原タンパク質をフローサイトメトリーで検証し、CARの発現が高い特異性で検出できます。ロット間の一貫性が保証されています。また、PE / FITC標識タンパク質を利用することで、ワンステップ染色でCARの発現を高い特異性でバックグラウンドなしに検出できます。
CD20、Claudin18.2、CD133、GPRC5D、CCR5、CCR8などの安定で高活性な全長型膜貫通タンパク質は、免疫学、ELISA、SPR、BLI、細胞実験、CAR陽性率検出に利用できます。VLP、界面活性剤、ナノディスクなどのテクニカルプラットフォームによって複数回膜貫通タンパク質の医薬品創薬を促進できます。
GMP準拠サイトカイン、高品質の細胞活性化/増幅試薬、遺伝子改変試薬/酵素、CAR検出試薬などの製品を提供し、細胞、遺伝子治療用に全体的なソリューションを提供し、創薬から臨床研究までのすべての段階をサポートします。
これまでに知られている免疫チェックポイント分子をほぼ製品化しており、様々なタグの製品群を提供できます。天然高分子の構造はMALSによって、生物活性はELISA/SPR/BLI/FACSなどによって検証済みです。またBiotin/FITCなどの標識も選択でき、抗体のハイスループットスクリーニングに利用できます。
弊社ACROBiosystemsはADC医薬品の開発のサポートに取り組み、注目されている様々なターゲットのために、異なる種類とタグの製品が開発され、高い純度と親和性が特長です。免疫、抗体スクリーン、SPR、細胞活性検査などの実験に利用できます。Protocolも無償で提供いたします。
すべての分子のFc受容体タンパク質だけでなく、一般的な変異体やビオチン標識タイプも含まれております。お客様のモノクローナル抗体の開発をサポートします。
インターロイキン、成長因子、ケモカイン、TNF など、様々なサイトカインターゲットの自然なコンフォメーションを確保するために HEK293 によって発現されます。SDS-PAGE/HPLC/SEC-MALS によって高純度は確認され、高い生物活性は ELISA/SPR/BLI によって確認されています。
AneuroはACROBiosystemsが神経科学研究のために設計した製品群のブランドです。神経科学研究を推進するための治療・診断用研究タンパク質、PFF、組み換え神経因子など、高品質の重要なタンパク質を提供しております。
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