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Your Position: ホーム > Protein > FcRn (FCGRT & B2M) > FCM-C82W9

Biotinylated Canine FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™&Tag Free (MALS & SPR verified)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Biotinylated Canine FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free(FCM-C82W9) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Pro 298 & Val 28 - Asn 125 (Accession # XP_038512242.1 & NP_001271408.1).
    Predicted N-terminus: Ala 23 | Val 28
  • Molecular Characterization
    FcRn (FCGRT & B2M) Structure

    This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 28.3 kDa | 11.5 kDa. The protein migrates as <14.4 kDa and 38-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FcRn (FCGRT & B2M) SDS-PAGE

Biotinylated Canine FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
FcRn (FCGRT & B2M) MALS images

The purity of Biotinylated Canine FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (Cat. No. FCM-C82W9) is more than 95% and the molecular weight of this protein is around 43-53 kDa verified by SEC-MALS.

Bioactivity-ELISA
 FcRn (FCGRT & B2M) ELISA

Immobilized Monoclonal Anti-Human CTLA-4 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Canine FcRn / FCGRT&B2M Heterodimer, His,Avitag&Tag Free (Cat. No. FCM-C82W9) with a linear range of 0.039-2.5 μg/mL (Routinely tested).

Bioactivity-SPR
 FcRn (FCGRT & B2M) SPR

Biotinylated Canine FCGRT&B2M Heterodimer, His,Avitag&Tag Free (Cat. No. FCM-C82W9) immobilized on SA Chip can bind Ipilimumab with an affinity constant of 42.5 nM as determined in a SPR assay (Biacore 8K) (QC tested).

 FcRn (FCGRT & B2M) SPR

Biotinylated Canine FCGRT&B2M Heterodimer, His,Avitag&Tag Free (Cat. No. FCM-C82W9) captured on CM5 chip via anti-His antibody can bind Ipilimumab with an affinity constant of 0.267 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

 FcRn (FCGRT & B2M) SPR

Biotinylated Canine FCGRT&B2M Heterodimer, His,Avitag&Tag Free (Cat. No. FCM-C82W9) immobilized on SA Chip can bind Trastuzumab with an affinity constant of 44.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • Clinical and Translational Updates

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