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Your Position: ホーム > Antibody > His > HIS-PLM535

HRP conjugated Anti-His Antibody (AY63), mAb

  • Source

    HRP conjugated Anti-His Antibody (AY63), mAb is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.

  • Clone

    AY63

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    HRP-Conjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Tag

  • Immunogen

    A protein peptide with his tag.

  • Specificity

    This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

  • Application
    ApplicationRecommended Usage
    Western Blot0.2-2 ug/mL 
    ELISA1:3000-1:10000
  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70 °C for 6 months after reconstitution.
    3. 2-8 °C for 2-3 weeks under sterile conditions after reconstitution.
Bioactivity-ELISA
 His ELISA

Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind HRP conjugated Anti-His Antibody (AY63), mAb (Cat. No. HIS-PLM535) with a linear range of 0.2-13 ng/mL (QC tested).

Western Blot
 His WESTERN BLOT

Detection of HRP conjugated Anti-His Tag Antibody (AY63), mAb by Western Blot. HRP conjugated Anti-His Antibody (AY63), mAb at 1/10000 dilution + Human LAG-3 / CD223 Protein, His Tag at 75-250ng.
Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.

  • Background
    His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 63,000

価格(JPY) : 423,360

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