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Anti-SARS-CoV-2 Spike Antibody, Mouse IgG1 (2C9C9) (XBB.1.5/Omicron Specific)

  • Source

    Anti-SARS-CoV-2 Spike Antibody, Mouse IgG1 (2C9C9) (XBB.1.5/Omicron Specific) is isolated from a Spike protein infected Mouse and is recombinantly produced from Hybridoma

  • Clone

    2C9C9

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Hybridoma Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant SARS-CoV-2 Spike Trimer Protein (XBB.1.5/Omicron) erived from human 293 cells.

  • Specificity

    This product is a specific antibody specifically reacts with SARS-CoV-2 Spike Trimer Protein, His Tag (XBB/Omicron) (MALS verified) (Cat. No. SPN-C5248), SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1/Omicron) (MALS verified) (Cat. No. SPN-C522t) and SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1.5/Omicron) (MALS verified) (Cat. No. SPN-C524i). No cross-reactivity is detected with Protein of WT (Cat. No. SPN-C52H9), Alpha (Cat. No. SPN-C52H6), Beta, Gamma (Cat. No. SPN-C52Hg), Delta (Cat. No. SPN-C52He), B.1.1.529/Omicron (Cat. No. SPN-C52Hz), BA.2/Omicron (Cat. No. SPN-C5223), BA.3/Omicron (Cat. No. SPN-C5225), BA.4/Omicron (Cat. No. SPN-C5229), BA.5/Omicron (Cat. No. SPN-C522e), BA.2.12.1/Omicron (Cat. No. SPN-C522d), BQ.1.1/Omicron (Cat. No. SPN-C522s), BA.2.75/Omicron (Cat. No. SPN-C522f), BA.4.6/Omicron (Cat. No. SPN-C522m), BF.7/Omicron (Cat. No. SPN-C522q).

  • Application
    ApplicationRecommended Usage
    ELISA0.8-200 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike protein SDS-PAGE

Anti-SARS-CoV-2 Spike Antibody, Mouse IgG1 (2C9C9) (XBB.1.5/Omicron Specific) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Spike protein ELISA

Immobilized SARS-CoV-2 Spike Trimer Protein, His Tag (XBB/Omicron) (MALS verified) (Cat. No. SPN-C5248), SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1/Omicron) (MALS verified) (Cat. No. SPN-C522t) and SARS-CoV-2 Spike Trimer Protein, His Tag (XBB.1.5/Omicron) (MALS verified) (Cat. No. SPN-C524i) can bind Anti-SARS-CoV-2 Spike Antibody, Mouse IgG1 (2C9C9) (XBB.1.5/Omicron Specific) (Cat. No. SPN-Y169) with a linear range of 0.391-25 ng/mL (QC tested). The antibody does not bind Spike Protein of WT (Cat. No. SPN-C52H9), Alpha (Cat. No. SPN-C52H6), Beta, Gamma (Cat. No. SPN-C52Hg), Delta (Cat. No. SPN-C52He), B.1.1.529/Omicron (Cat. No. SPN-C52Hz), BA.2/Omicron (Cat. No. SPN-C5223), BA.3/Omicron (Cat. No. SPN-C5225), BA.4/Omicron (Cat. No. SPN-C5229), BA.5/Omicron (Cat. No. SPN-C522e), BA.2.12.1/Omicron (Cat. No. SPN-C522d), BQ.1.1/Omicron (Cat. No. SPN-C522s), BA.2.75/Omicron (Cat. No. SPN-C522f), BA.4.6/Omicron (Cat. No. SPN-C522m), BF.7/Omicron (Cat. No. SPN-C522q) (QC tested).

  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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