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Your Position: ホーム > Protein > Envelope protein E/E protein (TBEV) > ENE-T52H4

TBEV (European subtype, strain Neudoerfl) Envelope protein E, His Tag (HPLC verified)

  • Source
    TBEV (European subtype, strain Neudoerfl) Envelope protein E, His Tag(ENE-T52H4) is expressed from human 293 cells (HEK293). It contains AA Ser 281-Lys 675 (Accession # P14336).
  • Molecular Characterization
    Envelope protein E/E protein (TBEV) Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 63.2 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Envelope protein E/E protein (TBEV) SDS-PAGE

TBEV (European subtype, strain Neudoerfl) Envelope protein E, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-HPLC
Envelope protein E/E protein (TBEV) SEC-HPLC

The purity of TBEV (European subtype, strain Neudoerfl) Envelope protein E, His Tag (Cat. No. ENE-T52H4) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
 Envelope protein E/E protein (TBEV) ELISA

Immobilized TBEV (European subtype, strain Neudoerfl) Envelope protein E, His Tag (Cat. No. ENE-T52H4) at 1 μg/mL (100 μL/well) can bind Anti-Envelope protein E, Tick-borne encephalitis virus European subtype (strain Neudoerfl) antibody with a linear range of 0.001-0.125 μg/mL (QC tested).

  • Background
    Tick-borne encephalitis virus (TBEV), which belongs to Family Flaviviridae, is the leading cause of Tick-borne encephalitis in human and animals across Europe and East Asia. The infection of TBE virus will lead to the illness called Tick-borne encephalitis with high morbidity and mortality. TBE virus has three regional subtypes of European subtype (TBEV-Eu), Far-eastern subtype TBEV (TBEV-FE) and Siberian subtype (TBEV-Sib) that dominate their respective regions; however, the sequence variety at amino acid level is quite low and the European subtypes’ vaccines show cross protection against other subtypes. The envelope protein E is the major viral surface antigen that plays an important role in viral infection, making it a great target for vaccine development.
  • Clinical and Translational Updates

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