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Your Position: ホーム > Protein > Insulin R > INR-H82E6

Biotinylated Human Insulin R / CD220 (28-944) Protein, His,Avitag™ (MALS & SPR verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    INSR,Insulin receptor,IR,CD220
  • Source
    Biotinylated Human Insulin R (28-944) Protein, His,Avitag(INR-H82E6) is expressed from human 293 cells (HEK293). It contains AA His 28 - Lys 944 (Accession # P06213-2).
    Predicted N-terminus: His 28
    Request for sequence
  • Molecular Characterization
    Insulin R Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 108.2 kDa. The protein migrates as 115-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Insulin R SDS-PAGE

Biotinylated Human Insulin R (28-944) Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Insulin R MALS images

The purity of Biotinylated Human Insulin R (28-944) Protein, His,Avitag (Cat. No. INR-H82E6) is more than 80% and the molecular weight of this protein is around 275-315 kDa verified by SEC-MALS.

Report
Bioactivity-SPR
Insulin R SPR

Biotinylated Human Insulin R (28-944) Protein, His,Avitag (Cat. No. INR-H82E6) immobilized on SA Chip can bind Insulin with an affinity constant of 35.6 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol
  • Background
    Insulin receptor (INSR) is also known as CD antigen CD220, which can be cleaved into the following 2 chains: Insulin receptor subunit alpha and Insulin receptor subunit beta. INSR is a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains carry the insulin-binding regions, while the beta chains carry the kinase domain. Forms a hybrid receptor with IGF1R, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short of INSR has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, INSR binds IGF1.
  • Clinical and Translational Updates

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