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Human FGF-17 Protein, His Tag (HPLC verified)

  • Synonym
    FGF-17,Fibroblast growth factor 17,FGF17
  • Source
    Human FGF-17 Protein, His Tag(FG7-H5144) is expressed from E. coli cells. It contains AA Thr 23 - Thr 216 (Accession # O60258-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    FGF-17 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 24.5 kDa. The protein migrates as 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Host Cell Protein
    <0.5 ng/µg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Sterility
    Negative
  • Mycoplasma
    Negative.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FGF-17 SDS-PAGE

Human FGF-17 Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-HPLC
FGF-17 SEC-HPLC

The purity of Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
 FGF-17 ELISA

Immobilized Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) with a linear range of 2-40 ng/mL (QC tested).

 FGF-17 ELISA

Immobilized Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) at 5 μg/mL (100 μL/well) can bind Human FGF R4, Fc Tag (Cat. No. FG4-H5253) with a linear range of 0.078-0.625 μg/mL (QC tested).

Bioactivity-Bioactivity CELL BASE
 FGF-17 CELL

Human FGF-17 Protein, His Tag (Cat. No. FG7-H5144) stimulates proliferation of NIH-3T3 cells. The EC50 for this effect is 1.495-1.639 µg/mL (Routinely tested).

  • Background
    Fibroblast growth factors (FGFs) are a large family of structurally related proteins that are involved in wide variety of cellular processes including proliferation, differentiation, migration, and apoptosis. FGF17 also referred to as FGF-13, is expressed during embryogenesis and in the adult cerebellum and cortex and may be essential for vascular growth and normal brain development. Additionally, FGF17 together with FGF8, is a key factor in the patterning of the mid-hindbrain region with a complex picture of spatiotemporal gene expression during the various stages of cerebellar development.
  • Clinical and Translational Updates

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