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Your Position: ホーム > Protein > IL-4 R alpha & IL-13 R alpha 1 > ILR-H525b

Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (MALS verified)

  • Synonym
    IL-4 R alpha & IL-13 R alpha 1, IL4RA & IL13RA1
  • Source
    Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag(ILR-H525b) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 & Gly 22 - Thr 343 (Accession # P24394-1 & NP_001551.1).
    Predicted N-terminus: Met 26 & Gly 22
  • Molecular Characterization
    IL-4 R alpha & IL-13 R alpha 1 Structure

    Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag is produced by co-expression of IL-4 R alpha and IL-13 R alpha 1, has a calculated MW of 50.2 kDa & 63.1 kDa. The protein migrates as 65-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-4 R alpha & IL-13 R alpha 1 SDS-PAGE

Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-4 R alpha & IL-13 R alpha 1 MALS images

The purity of Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (Cat. No. ILR-H525b) is more than 90% and the molecular weight of this protein is around 140-170 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-4 R alpha & IL-13 R alpha 1 ELISA

Immobilized Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (Cat. No. ILR-H525b) at 5 μg/mL (100 μL/well) can bind Human IL-4, His Tag (Cat. No. IL4-H52H9) with a linear range of 0.6-5 ng/mL (QC tested).

 IL-4 R alpha & IL-13 R alpha 1 ELISA

Immobilized Human IL-13 Protein, His Tag (Cat. No. IL3-H52H4) at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (Cat. No. ILR-H525b) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

  • Background
    Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.
  • Clinical and Translational Updates

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