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Your Position: ホーム > Protein > HLA-A*0201 & B2M & PRAME (SLLQHLIGL) > HLE-H52H9

Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (MALS verified)

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!
  • Synonym
    HLA-A*0201 & B2M & PRAME (SLLQHLIGL)
  • Source
    Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein(HLE-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Met 119 (B2M) & Gly 25 - Ile 308 (HLA-A*02:01) & SLLQHLIGL peptide (Accession # P61769 (B2M) & AAA59606.1 (HLA-A*02:01) & SLLQHLIGL).
  • Molecular Characterization

    Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein is assembled by biotinylated monomer (HLE-H82E8) and streptavidin.

    Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Complex Protein is produced by co-expression of HLA and B2M loaded with PRAME peptide. Biotinylated Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 51.3 kDa and 13.8 kDa. The protein migrates as 55-60 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*0201 & B2M & PRAME (SLLQHLIGL) SDS-PAGE

Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
HLA-A*0201 & B2M & PRAME (SLLQHLIGL) MALS images

The purity of Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (Cat. No. HLE-H52H9) is more than 90% and the molecular weight of this protein is around 270-295 kDa verified by SEC-MALS.

Bioactivity-ELISA
 HLA-A*0201 & B2M & PRAME (SLLQHLIGL) ELISA

Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (Cat. No. HLE-H52H9) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).

  • Background
    This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. The PRAME (SLLQHLIGL) was shown to be recognized by HLA-A*0201 tumor-infiltrating lymphocytes from melanoma patients, and therefore it is widely been studied in TCR-T studies. The Human HLA-A*0201 PRAME (SLLQHLIGL) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M and PRAME (SLLQHLIGL) peptide.
  • Clinical and Translational Updates

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価格(JPY) : 448,000

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