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Your Position: ホーム > Protein > IL-7 R alpha & IL-2 R gamma > ILA-H82W3

Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, His,Avitag™&Tag Free (MALS verified)

  • Source
    Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, His,Avitag&Tag Free(ILA-H82W3)  is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Asp 239 & Leu 23 - Asn 254 (Accession # P16871-1  & P31785-1).
    Predicted N-terminus: Glu 21 & Leu 23
  • Molecular Characterization
    IL-7 R alpha & IL-2 R gamma Structure

    Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein has a calculated MW of 32.2 kDa (IL-7 R alpha) & 31.2 kDa (IL-2 R gamma). The protein migrates as 43-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
IL-7 R alpha & IL-2 R gamma SDS-PAGE

Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-7 R alpha & IL-2 R gamma MALS images

The purity of Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, His,Avitag&Tag Free (Cat. No. ILA-H82W3) is more than 90% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-7 R alpha & IL-2 R gamma ELISA

Immobilized Human IL-7, Fc Tag (Cat. No. IL7-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, His,Avitag&Tag Free (Cat. No. ILA-H82W3) with a linear range of 0.1-2 ng/mL (QC tested).

  • Background
    The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found.
  • Clinical and Translational Updates

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