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Your Position: ホーム > Protein > HLA-A*1101 & B2M & WT1 (FSCRWPSCQK) > HLW-HA2H4

Alexa Fluor™ 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer ProteinStar Staining

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!
  • Source
    Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein(HLW-HA2H4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & FSCRWPSCQK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & FSCRWPSCQK).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein is assembled by biotinylated monomer and AF647-labeled streptavidin.

    Biotinylated Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Complex Protein is produced by co-expression of HLA and B2M loaded with WT-1 peptide. Biotinylated Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • Conjugate

    AF647

    Excitation Wavelength: 640 nm

    Emission Wavelength: 672 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-ELISA
 HLA-A*1101 & B2M & WT1 (FSCRWPSCQK) ELISA

Immobilized Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein (Cat. No. HLW-HA2H4) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.12-2 ng/mL (QC tested).

 HLA-A*1101 & B2M & WT1 (FSCRWPSCQK) ELISA

Immobilized Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein (Cat. No. HLW-HA2H4) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.06-1 ng/mL (Routinely tested).

  • Background
    The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Biotinylated Human HLA-A*1101 WT1 (FSCRWPSCQK) complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and FSCRWPSCQK peptide of the KRAS.
  • Clinical and Translational Updates

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