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Human Kallikrein 3 Protein, His Tag (active enzyme)

  • Synonym
    KLK3,Kallikrein-3,APS,KLK2A1,PSA,Hk3,Seminin,Semenogelase
  • Source
    Human Kallikrein 3 Protein, His Tag(KL3-H52H3) is expressed from human 293 cells (HEK293).
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.7 kDa. The protein migrates as 33-36 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Kallikrein 3 SDS-PAGE

Human Kallikrein 3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity

Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-R-P-Y-pNA). The specific activity is >150 pmol/min/µg (QC tested).

  • Background
    Kallikrein-3 (KLK3) is also known as Prostate-specific antigen (PSA), APS, Gamma-seminoprotein (Seminin), P-30 antigen, Semenogelase, is a glycoprotein enzyme, is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. The preferential cleavage site of KLK3 is “-Tyr-|-Xaa-“, the catalytic activity is inhibited by SERPINA5 and is strongly inhibited by Zn2+. KLK3 hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Clinical and Translational Updates

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