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AAV5 VP2, Recombinant Protein

  • Synonym
    VP2
  • Source
    AAV5 VP2, Recombinant Protein(VP2-A5144) is expressed from E. coli cells. It contains AA Thr 137 - Leu 724 (Accession # Q9YIJ1-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    VP2 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 67.4 kDa. The protein migrates as 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 6 M Urea, PBS, pH7.3 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
VP2 SDS-PAGE

AAV5 VP2, Recombinant Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Background
    Adeno-associated virus is a single-stranded DNA virus and the current scientific consensus is that it does not cause any human disease. It consists of a protein capsid (CAPside) and a 4.7 KB length single stranded DNA genome. The protein capsid consists of three subunits, VP1, VP2, and VP3.
  • Clinical and Translational Updates

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