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Your Position: ホーム > Protein > B7-H3 > B73-HP2H3

PE-Labeled Human B7-H3 / CD276 Protein, His Tag

  • Synonym
    B7-H3,CD276,B7 homolog 3
  • Source
    PE-Labeled Human B7-H3 Protein, His Tag(B73-HP2H3) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Pro 245 (Accession # Q5ZPR3-2).
    Predicted N-terminus: Leu 29
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 37.6 kDa.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
Evaluation of CAR expression
 B7-H3 CAR_T

5e5 of anti-B7-H3 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human B7-H3 Protein, His Tag (Cat. No. B73-HP2H3) and negative control protein respectively(Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity (QC tested).

 B7-H3 CAR_T

5e5 of PBMCs were stained with PE-Labeled Human B7-H3 Protein, His Tag (Cat. No. B73-HP2H3) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs , and PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

  • Background
    B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.
  • Clinical and Translational Updates

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