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Human CNTF R alpha Protein, His Tag

  • Synonym
    CNTFR,CNTF R alpha
  • Source
    Human CNTF R alpha, His Tag(CNR-H5225) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Pro 346 (Accession # AAH01492 ).
    Predicted N-terminus: Gln 23
  • Molecular Characterization
    CNTF R alpha Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 37.6 kDa. The protein migrates as 45-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CNTF R alpha SDS-PAGE

Human CNTF R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Ciliary neurotrophic factor receptor subunit alpha (CNTFR or CNTFR-alpha) belongs to the type I cytokine receptor family and type 3 subfamily. CNTFR contains two fibronectin type-III domains and one Ig-like C2-type (immunoglobulin-like) domain. This receptor and its cognate ligand support the survival of neurons. This receptor is most closely related to the interleukin-6 receptor. This receptor possesses an unusual attachment to the cell membrane through a glycophosphatidylinositol linkage.
  • Clinical and Translational Updates

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