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Your Position: ホーム > Protein > CD27 > CD7-M5252

Mouse CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CD27,TNFRSF7,S152,T14,Tp55
  • Source
    Mouse CD27 Protein, Mouse IgG2a Fc Tag(CD7-M5252) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Arg 182 (Accession # P41272-1).
    Predicted N-terminus: Thr 21
  • Molecular Characterization
    CD27 Structure

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 45.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD27 SDS-PAGE

Mouse CD27 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 CD27 ELISA

Immobilized Mouse CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse CD70, His Tag, Avi Tag with a linear range of 1-16 ng/mL (QC tested).

 CD27 ELISA

Immobilized Mouse CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand Protein, Fc Tag with a linear range of 1-16 ng/mL (Routinely tested).

  • Background
    CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 98,000

価格(JPY) : 711,200

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