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Your Position: ホーム > Protein > TROP-2 > TR2-H82E5

Biotinylated Human TROP-2 / TACSTD2 Protein, His,Avitag™ (MALS verified)

  • Synonym
    TACSTD2,GA733-1,M1S1,TROP2
  • Source
    Biotinylated Human TROP-2, His,Avitag(TR2-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 274 (Accession # P09758-1).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    TROP-2 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 31.2 kDa. The protein migrates as 42-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TROP-2 SDS-PAGE

Biotinylated Human TROP-2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
TROP-2 MALS images

The purity of Biotinylated Human TROP-2, His,Avitag (Cat. No. TR2-H82E5) is more than 90% and the molecular weight of this protein is around 32-47 kDa verified by SEC-MALS.

Bioactivity-ELISA
 TROP-2 ELISA

Immobilized Biotinylated Human TROP-2, His,Avitag (Cat. No. TR2-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Monoclonal Antibody Against Human TROP-2, Mouse IgG1 with a linear range of 0.5-4 ng/mL (QC tested).

Bioactivity-SPR
 TROP-2 SPR

Captured Trop2 antibody on CM5 chip via anti-mouse antibodies surface can bind Biotinylated Human TROP-2, His,Avitag (Cat. No. TR2-H82E5) with an affinity constant of 6.35 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Background
    TROP-2 is a single-copy gene in human cells, and encodes a type-1 transmembrane glycoprotein which is over-expressed in various malignancies, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1. TROP-2 is related to epithelial cell adhesion molecule (EpCAM), also called TROP-1, gp40, and KSA. Trop-1 and Trop-2 are homologous to serum IGF-II-binding proteins and appear as signal transducers. Thus, they likely represent novel cell-surface receptors and may play a role in regulating the growth of carcinoma cells.
  • Clinical and Translational Updates

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