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Your Position: ホーム > Antibody > Bevacizumab > BEB-Y10

Anti-Bevacizumab Antibody (AY10) (MALS verified, recommended for PK/PD)

  • Source

    Anti-Bevacizumab Antibody (AY10) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.

  • Clone

    AY10

  • Species

    Mouse

  • Isotype

    Mouse IgG1/kappa

  • Antibody Type

    Hybridoma Monoclonal

  • Reactivity

    Human

  • Immunogen

    Bevacizumab.

  • Specificity

    Recognizes Bevacizumab specifically.

  • Application
    ApplicationRecommended Usage
    ELISA10-20000 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Bevacizumab SDS-PAGE

Anti-Bevacizumab Antibody (AY10) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Bevacizumab MALS images

The purity of Anti-Bevacizumab Antibody (AY10) (Cat. No. BEB-Y10) is more than 95% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Bevacizumab ELISA

Detection of bevacizumab by bridging ELISA in serum. Immobilized Anti-Bevacizumab Antibody (AY10) (Cat. No. BEB-Y10) at 2 μg/mL, add increasing concentrations of bevacizumab (10% human serum) and then add Biotinylated Anti-Bevacizumab Antibody (AY13) (Cat. No. BEB-BY13) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 0.4 μg/mL (QC tested).

 Bevacizumab ELISA

Immobilized Bevacizumab at 1 μg/mL (100 μL/well) can bind Anti-Bevacizumab Antibody (AY10) (Cat. No. BEB-Y10) with a linear range of 0.39-25 ng/mL.

 Bevacizumab ELISA

Immobilized Bevacizumab at 2 μg/mL (100 μL/well) can bind pre-mixed Anti-Bevacizumab Antibody (AY10) (Cat. No. BEB-Y10) and Biotinylated Human VEGF165, His,Avitag (Cat. No. VE5-H82Q0) with a inhibition rate of 81%.

  • Background
    A recombinant humanized monoclonal IgG1 antibody that binds to and inhibits the biologic activity of human vascular endothelial growth factor (VEGF). Bevacizumab contains human framework regions and the complementarity-determining regions of a murine antibody that binds to VEGF. Bevacizumab is produced in a Chinese Hamster Ovary mammalian cell expression system in a nutrient medium containing the antibiotic gentamicin and has a molecular weight of approximately 149 kilodaltons.
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(USD) : 300.00

価格(USD) : 2180.00

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