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Your Position: ホーム > Protein > Nectin-1 > PV1-H5253

Human Nectin-1 / PVRL1 / CD111 Protein, Fc Tag

  • Synonym
    PVRL1,Nectin-1,CD111,CLPED1,ED4,HIgR,HVEC,OFC7,PRR,PRR1,PVRR,PVRR1,SK-12
  • Source
    Human Nectin-1, Fc Tag(PV1-H5253) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 334 (Accession # Q15223-1).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    Nectin-1 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 60.4 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Nectin-1 SDS-PAGE

Human Nectin-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 Nectin-1 ELISA

Immobilized Human Nectin-3, His Tag (Cat. No. PV3-H52E4) at 5 μg/mL (100 μL/well) can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with a linear range of 1-39 ng/mL (QC tested).

 Nectin-1 ELISA

Immobilized Biotinylated Human Nectin-4, His,Avitag (Cat. No. NE4-H82E7) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with a linear range of 2-40 ng/mL (Routinely tested).

Bioactivity-BLI
 Nectin-1 BLI

Loaded Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) on Protein A Biosensor, can bind Human Nectin-4, His Tag (Cat. No. NE4-H52H3) with an affinity constant of 0.271 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Nectin-1 BLI

Loaded Cynomolgus Nectin-4, His Tag (Cat. No. NE4-C52H4) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 0.23 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Nectin-1 BLI

Loaded Mouse Nectin-4, His Tag (Cat. No. NE4-M52H3) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 0.12 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Nectin-1 BLI

Loaded Human Nectin-3, His Tag (Cat. No. PV3-H52E4) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 4.99 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Poliovirus receptor-related protein 1 (PVRL1) is also known as Herpes virus entry mediator C (HveC), Herpesvirus Ig-like receptor (HIgR), Nectin-1, CD antigen CD111, PVRL1 / Nectin-1 / CD111 belongs to the nectin family. PVRL1 / Nectin-1 is a membrane protein with three extracellular immunoglobulin domains, a single transmembrane helix and a cytoplasmic tail. The protein can mediate Ca2+-independent cellular adhesion further characterizing it as IgSF cell adhesion molecule (IgSF CAM). PVRL1 is adhesion molecule found in a wide range of tissues. PVRL1 / Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4.
  • Clinical and Translational Updates

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価格(USD) : 2180.00

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