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Your Position: ホーム > Protein > PTK7 > PT7-H82E5

Biotinylated Human PTK7 / CCK4 protein, His,Avitag™ (MALS verified)

  • Synonym
    PTK7,CCK4,CCK-4,Tyrosine-protein kinase-like 7,Protein-tyrosine kinase 7,Protein-tyrosine kinase 7
  • Source
    Biotinylated Human PTK7, His,Avitag(PT7-H82E5) is expressed from human 293 cells (HEK293). It contains AA Ala 31 - Thr 704 (Accession # Q13308-1).
    Predicted N-terminus: Ala 31
  • Molecular Characterization
    PTK7 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 78.2 kDa. The protein migrates as 105-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
PTK7 SDS-PAGE

Biotinylated Human PTK7, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
PTK7 MALS images

The purity of Biotinylated Human PTK7, His,Avitag (Cat. No. PT7-H82E5) is more than 90% and the molecular weight of this protein is around 115-130 kDa verified by SEC-MALS.

Bioactivity-ELISA
 PTK7 ELISA

Immobilized Biotinylated Human PTK7, His,Avitag (Cat. No. PT7-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Cofetuzumab Pelidotin with a linear range of 0.06-1 ng/mL (Routinely tested).

  • Background
    PTK7, highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
  • Clinical and Translational Updates

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