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Your Position: ホーム > Protein > ALPL > ALL-H52H3

Human ALPL Protein, His Tag (active enzyme, MALS verified)

  • Synonym
    AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP
  • Source
    Human ALPL Protein, His Tag(ALL-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 18 - Ser 502 (Accession # P05186-1).
    Predicted N-terminus: Leu 18
  • Molecular Characterization
    ALPL Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 55.3 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, 20% Glycerol, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
ALPL SDS-PAGE

Human ALPL Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
ALPL MALS images

The purity of Human ALPL Protein, His Tag (Cat. No. ALL-H52H3) is more than 90% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.

Bioactivity-ELISA
 ALPL ELISA

Immobilized Human ALPL Protein, His Tag (Cat. No. ALL-H52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-ALPL Polyclonal Antibody (QC tested).

Bioactivity

Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >80,000 pmol/min/μg (QC tested).

  • Background
    This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 106,400

価格(JPY) : 728,000

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