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ClinMax™ Human MCP-1 / CCL2 ELISA Kit

For research use only.
    Product Details
    ClinMax™ Human MCP-1/CCL2 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human CCL2 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
  • Application

    The kit is developed for quantitative detection of natural and recombinant human CCL2 in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow
     CCL2 Workflow
    Key Features
    AnalyteMCP-1
    Assay TypeSandwich-ELISA
    ReactivityHuman
    Sensitivity2pg/mL
    Range4.688 pg/mL-300 pg/mL
    Assay Time3hr
    Sample TypeCell Culture Supernatants, Plasma, Serum.
    Sample volume50 μL
    Format96-wells plate breakable into 12 x 8 wells strips

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
 CCL2 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of MCP-1 is less than 2 pg/mL.

Validation
Recovery

Recombinant MCP-1 was spiked into 3 human serum samples, and then analyzed. The average recovery of MCP-1 for serum samples is 108%.

 CCL2 RECOVERY
Materials Provided
IDComponentsSize
CEA036-C01Pre-coated Anti-MCP-1 Antibody Microplate1 plate
CEA036-C02Human MCP-1 Standard150 ng×2
CEA036-C03Biotin-Anti-MCP-1 Antibody Con. Solution150 μL
CEA036-C04Biotin-Antibody Dilution Buffer8 mL
CEA036-C05Streptavidin-HRP Con. Solution500 μL
CEA036-C06Streptavidin-HRP Dilution Buffer15 mL
CEA036-C0720× Washing Buffer50 mL
CEA036-C08Sample Dilution Buffer15 mL ×2
CEA036-C09Substrate Solution12 mL
CEA036-C10Stop Solution6 mL
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 153,720

価格(JPY) : 768,600

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