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ClinMax™ Human Soluble BCMA ELISA Kit

For research use only.
    Product Details
    ClinMax™ Human Soluble BCMA ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human BCMA that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
    1. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    2. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    3. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
  • Application

    The kit is developed for quantitative detection of natural and recombinant human BCMA in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow
     BCMA Workflow
    Key Features
    AnalyteSoluble BCMA
    Assay TypeSandwich-ELISA
    ReactivityHuman
    Sensitivity150 pg/mL
    Range375 pg/mL-24000 pg/mL
    Assay Time2 hr 45 min
    Sample TypeCell Culture Supernatants, Plasma, Serum.
    Sample volume50 uL
    Format96-wells plate breakable into 12 x 8 wells strips

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
 BCMA TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of BCMA is less than 150 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 3 samples containing different BCMA concentrations were tested in one assay. Acceptable criteria: CV<10%.

 BCMA INTRA-ASSAY STATISTICS
Inter-Assay Statistics

3 samples containing different concentrations of BCMA were tested in independent assays. Acceptable criteria: CV<15%.

 BCMA INTER-ASSAY STATISTICS
Recovery

Recombinant BCMA was spiked into 3 human serum samples, and then analyzed. The average recovery of BCMA for serum samples is 97.4%.

 BCMA RECOVERY
Materials Provided
IDComponentsSize
CEA045-C01Pre-coated Anti-Soluble BCMA Antibody Microplate1 plate
CEA045-C02Human Soluble BCMA Standard320 μg×2
CEA045-C03Biotin-Anti-Soluble BCMA Antibody Con. Solution100 μL
CEA045-C04Biotin-Antibody Dilution Buffer8 mL
CEA045-C05Streptavidin-HRP Con. Solution500 μL
CEA045-C06Streptavidin-HRP Dilution Buffer15 mL
CEA045-C0720× Washing Buffer50 mL
CEA045-C08Sample Dilution Buffer15 mL×2
CEA045-C09Substrate Solution12 mL
CEA045-C10Stop Solution6 mL
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 153,440

価格(JPY) : 690,480

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